An automated imaging system for radiation biodosimetry

An automated imaging system for radiation biodosimetry.

We describe here an automated imaging system developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry. The imaging system is built around a fast, sensitive sCMOS camera and rapid switchable LED light source. It features complete automation of all the steps of the imaging process and contains built-in feedback loops to ensure proper operation. The imaging system is i...

Radiation Dose Estimation by Automated Cytogenetic Biodosimetry.

The dose from ionizing radiation exposure can be interpolated from a calibration curve fit to the frequency of dicentric chromosomes (DCs) at multiple doses. As DC counts are manually determined, there is an acute need for accurate, fully automated biodosimetry calibration curve generation and analysis of exposed samples. Software, the Automated Dicentric Chromosome Identifier (ADCI), is presen...

Optimized automated data analysis for the cytokinesis‐block micronucleus assay using imaging flow cytometry for high throughput radiation biodosimetry

The cytokinesis-block micronucleus (CBMN) assay is a well-established technique that can be employed in triage radiation biodosimetry to estimate whole body doses of radiation to potentially exposed individuals through quantitation of the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay has been partially automated using traditional microscope-based methods and mo...

developing an automated cytogenetic imaging system for detection of dicentric chromosomes in biological dosimetry

background: high-energy ionizing radiation is harmful and changes the genetic makeup of dna, which can lead to increased risk of cancer. thus, the exposure of radiation dose should be under control and limited. ionizing radiation might lead to some chromosome aberrations like dicentric. there is a strong relation between the frequency of dicentric chromosome in metaphase, and the received dose....

Serum microRNAs – potent biomarkers for radiation biodosimetry